Light-dependent absorption and selective scattering changes at 518 nm in chloroplast thylakoid membranes.

Light-dependent absorption and selective scattering changes at 518 nm in chloroplast thylakoid membranes.

The light-induced absorbance change at 518 nm of isolated chloroplasts consists of a rapid phase, and a slow phase which is complete in about 20 sec. The slow component of the 518 nm absorbance change correlates with the light-induced change in 90 degrees light scattering at 518 nm. Both show a similar time course, similar pH dependence with a maximum at pH 6.0, and similar sensitivity to inhib...

Light-dependent redistribution of ions in suspensions of chloroplast thylakoid membranes.

Ion movements associated with the pH rise that is observed upon illumination of thylakoid suspensions at low pH have been studied by a multiparameter technique. Light-dependent, dark-reversible fluxes of H(+), Cl(-), Na(+), K(+) and divalent cations were monitored, together with simultaneous changes in the optical density of the suspension. Extensive uptake of Cl(-) and efflux of Mg(2+) accompa...

Optical Absorption of Silicon Nitride Membranes at 1064 nm and at 1550 nm

Low-pH induced reversible reorganizations of chloroplast thylakoid membranes - As revealed by small-angle neutron scattering.

Energization of thylakoid membranes brings about the acidification of the lumenal aqueous phase, which activates important regulatory mechanisms. Earlier Jajoo and coworkers (2014 FEBS Lett. 588:970) have shown that low pH in isolated plant thylakoid membranes induces changes in the excitation energy distribution between the two photosystems. In order to elucidate the structural background of t...

Attachment of chloroplast polysomes to thylakoid membranes in Chlamydomonas reinhardtii.

Treatment of synchronous cultures of Chlamydomonas reinhardtii with chloramphenicol at 4 hr after the beginning of the light phase led to a preferential loss of 70S ribosomes from the 17,000 x g(max) supernate. The "lost" 70S ribosomes were found associated with a thylakoid membrane fraction prepared from the 17,000 x g(max) pellet. Electron microscopic examinations of this fraction revealed th...